Select those primers with one band in the gel picture and one peak in the dissociation curve for further experiments. In a one step rt pcr protocol multiscribe reverse transcriptase and rnase inhibitor are added to the sybr green pcr master mix. Quantitative rt pcr protocol sybr green i 5 3 running 3 agarose gel after qrt pcr 4 analyze qrt pcr data.
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